ME2 - Squadmate ability with Q or E requires you to set it with controller first
Unlike ME2 on Xbox, now that I am playing with ME2 LE on PC, my squad never immediately use abilities when I press Q or E. So I found a fix. Connect a controller and use "x" to set the default left and right d-pad abilities, then switch back to keyboard, and voila, you can use their abilities on command with Q or E on the keyboard from then on.
The way it should work, is Q or E should use a teammate's ability immediately, the one that you manually used last. Let's say you assign Miranda's Overload ability to "5" on the quick bar. What should happen is after you use it once, from then on, every time you press Q or E it should fire. I have to keep a controller connected to play the PC version properly, which is frustrating. Imagine how many people are giving squad orders right now with the PC version and it isn't working correctly, and they don't realize it.