Absolutely must have for the run to work: talzin lead and ventress unique.
Really, really, reeeaaaaalllly should have but not technically ‘required’: daka unique (but seriously just get it, save yourself the frustration, the runs without her zeta are far worse 3-4m vs 10m+)
Kinda nice to have but not needed: talzin unique
If you really want to waste a zeta: initiate unique (only adds a few hundred thousand and isn’t worth it)
A low gear initiate is generally seen as better, easier to kill off 3 times to help get the run going.